Biolog System
The Biolog Microbial Identification System offers a fast and easy way to identify more than 2200 species of both Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi. This system has excellent potential for the study of bacterial succession and dynamics even on the strain level.
Correspondingly, What is Biolog used for? The Biolog equipment is used for the identification of aerobic and anaerobic bacteria, yeasts and fungi, with importance in the oil & gas industry, allowing us to optimize our processes, obtaining more information related to the microorganisms under study with high quality results.
What is a Biolog test? Biolog’s carbon source utilization technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern or « metabolic fingerprint » from discrete test reactions performed within a 96 well microplate.
Furthermore, How long should an EcoPlate incubate after it’s inoculated?
Finally, you will inoculate the EcoPlate using the multichannel pipettor and incubate it for about four days at room temperature.
What are Biolog plates?
Biolog MicroPlates were originally developed for the rapid identification of bacterial isolates by sole-carbon source utilization, through the inoculation of 95 individual carbon sources plus a water control on a 96 well plate. The plates are read between 24 and 72 h following inoculation with a pre-grown isolate.
What is the principle of Vitek? PRINCIPLE: The Vitek 2 Compact (30 card capacity) system uses a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing using a 64 well card that is barcoded with information on card type, expiration date, lot number and unique card identification number.
How does Biolog EcoPlate work? The EcoPlate contains 31 carbon sources that are useful for community analysis. These 31 carbon sources are repeated 3 times to give the scientist more replicates of the data. Communities of microorganisms will give a characteristic reaction pattern called a metabolic fingerprint.
What is inoculating fluid? The inoculating fluid contains a redox dye that turns purple if the organism is actively metabolizing in the well. In columns 1-9, the carbon source is varied (A1 is the negative control) so that if the organism is able to utilize the particular compound present, a purple color will result.
How does an eco plate work?
Each EcoPlate is filled with a dilution of one soil suspension, thus representing one soil sample. The utilization rates of carbon compounds in the wells are quantified spectrophotometrically by following the reduction of water-soluble colourless triphenyl tetrazolium chloride to purple triphenyl formazan.
How do I use Vitek?
What is a Vitek card?
Cards for species-level identification
VITEK®2 identification (ID) cards let you easily and safely test and identify a wide range of clinically relevant organisms. Designed for VITEK®2 automated instruments, VITEK®2 ID cards help ensure you can rapidly provide accurate, relevant results.
How does Vitek 2 generate MIC values? How is the MIC determined? The MIC is determined by comparing the growth of the patient isolate to the growth of isolates with known MICs. It is like having a standard curve stored in the VITEK® 2, where the standard curve relates reference MICs to organism activity in the antibiotic wells.
How many wells are in an EcoPlate?
The EcoPlate™ includes three replicate wells containing 31 organic carbon substrates and a control well with redox-sensitive tetrazolium dye, but no substrate, for community-level physiological profiling (CLPP) of metabolically active heterotrophic bacterial assemblages able to grow in plate conditions [32].
How many different carbon substrates are there on the EcoPlate?
The EcoPlate is composed of 31 different carbon compounds divided into six categories along with the control wells in a 96-well microplate (Table 2).
How is AWCD calculated? For each soil sample and at each incubation time point, average well color development (AWCD) was calculated according to the equation:AWCD = [Σ (C – R)] / n where C represents the absorbance value of control wells (mean of 3 controls), R is the mean absorbance of the response wells (3 wells per carbon substrate), and …
What are inoculating tools? An inoculation loop, also called a smear loop, inoculation wand or microstreaker, is a simple tool used mainly by microbiologists to pick up and transfer a small sample (inoculum) from a culture of microorganisms, e.g. for streaking on a culture plate.
How is inoculation done?
inoculation, process of producing immunity and method of vaccination that consists of introduction of the infectious agent onto an abraded or absorptive skin surface instead of inserting the substance in the tissues by means of a hollow needle, as in injection.
How do you inoculate?
How do I set up Vitek?
How do I use my Vitek 2 Compact? The VITEK ® 2 COMPACT is simple to use:
- After primary organism isolation, there is minimal handling with a simple standardized inoculum.
- Place the inoculum into the VITEK ® 2 Cassette at the Smart Carrier Station. …
- The VITEK ® 2 Card and sample are linked via barcode.
What is BD Phoenix?
BD Phoenix™ automated identification and susceptibility testing system. The BD Phoenix™ automated identification and susceptibility testing system provides rapid, accurate and reliable detection of known and emerging antimicrobial resistance.
What are Vitek 2 cards? Ready-to-use, flexible VITEK® 2 AST cards are designed for use on the innovative, automated VITEK® 2 family of instruments. They provide AST results and resistance detection for clinically important Gram-positive cocci, Gram-negative bacilli and yeasts. The system is proven to be rapid, reliable and accurate.
What is the Vitek 2 instrument used for? Automated instrument for ID/AST testing
Combining an innovative, automated platform with an expansive database, the VITEK® 2 microbial ID/AST testing system offers the confidence of fast, accurate results.
Is Vitek 2 a broth Microdilution?
In contrast, the VITEK 2 colistin susceptibility test performed well in our study. We found a high level of agreement with the reference broth microdilution method. The main disadvantage of the VITEK 2 is its low sensitivity to detect resistant subpopulations of E.
How does Vitek 2 AST work?
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The VITEK® 2 AST card is essentially a miniaturized, abbreviated and automated version of the doubling dilution technique for MICs determined by the microdilution method. The automated growth-based detection uses attenuation of light measured by an optical scanner. Each AST card contains 64 microwells.
