Biolog phenotype assays achieve this by coupling tetrazolium dyes with minimally defined nutrients to measure the impact of hundreds of carbon, nitrogen, phosphorous, and sulfur sources on redox reactions that result from compound-induced effects on the electron transport chain.
Correspondingly, What is the principle of Vitek? PRINCIPLE: The Vitek 2 Compact (30 card capacity) system uses a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing using a 64 well card that is barcoded with information on card type, expiration date, lot number and unique card identification number.
What is an EcoPlate? An EcoPlate is a 96-well microplate that contains 31 common carbon sources from altogether five compound groups—that is, carbohydrates, carboxylic and ketonic acids, amines and amides, amino acids and polymers—plus a blank well as a control, all these replicated thrice to control variation in inoculum densities.
Furthermore, What is microbial identification?
Microbial identification can be defined as “microbial characterization by a limited spectrum of tests pre-chosen and appropriate to the problem being studied” [1].
How do I use Vitek?
What is the Vitek 2 instrument used for? Automated instrument for ID/AST testing
Combining an innovative, automated platform with an expansive database, the VITEK® 2 microbial ID/AST testing system offers the confidence of fast, accurate results.
What is a Vitek card? Cards for species-level identification
VITEK®2 identification (ID) cards let you easily and safely test and identify a wide range of clinically relevant organisms. Designed for VITEK®2 automated instruments, VITEK®2 ID cards help ensure you can rapidly provide accurate, relevant results.
How long should an EcoPlate incubate after it’s inoculated? Finally, you will inoculate the EcoPlate using the multichannel pipettor and incubate it for about four days at room temperature.
How many wells are in an EcoPlate?
The EcoPlate™ includes three replicate wells containing 31 organic carbon substrates and a control well with redox-sensitive tetrazolium dye, but no substrate, for community-level physiological profiling (CLPP) of metabolically active heterotrophic bacterial assemblages able to grow in plate conditions [32].
How is AWCD calculated? For each soil sample and at each incubation time point, average well color development (AWCD) was calculated according to the equation:AWCD = [Σ (C – R)] / n where C represents the absorbance value of control wells (mean of 3 controls), R is the mean absorbance of the response wells (3 wells per carbon substrate), and …
What are the 4 types of microbes?
The major groups of microorganisms—namely bacteria, archaea, fungi (yeasts and molds), algae, protozoa, and viruses—are summarized below.
What are 3 methods used to identify bacteria? When identifying bacteria in the laboratory, the following chatacteristics are used: Gram staining, shape, presence of a capsule, bonding tendency (singly or in pairs), motility, respiration, growth medium, and whether it is intra- or extracellular.
What are the two types of bacteria?
Types
- Spherical: Bacteria shaped like a ball are called cocci, and a single bacterium is a coccus. Examples include the streptococcus group, responsible for “strep throat.”
- Rod-shaped: These are known as bacilli (singular bacillus). …
- Spiral: These are known as spirilla (singular spirillus).
How do I set up Vitek?
How do I use my Vitek 2 Compact? The VITEK ® 2 COMPACT is simple to use:
- After primary organism isolation, there is minimal handling with a simple standardized inoculum.
- Place the inoculum into the VITEK ® 2 Cassette at the Smart Carrier Station. …
- The VITEK ® 2 Card and sample are linked via barcode.
What is BD Phoenix? BD Phoenix™ automated identification and susceptibility testing system. The BD Phoenix™ automated identification and susceptibility testing system provides rapid, accurate and reliable detection of known and emerging antimicrobial resistance.
What is the Kirby Bauer technique?
In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and wafers of antibiotics (white disks, shown) are added to the plate. After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
What is fluorogenic methodology? Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate or in cell suspensions.
What are Vitek 2 cards?
Ready-to-use, flexible VITEK® 2 AST cards are designed for use on the innovative, automated VITEK® 2 family of instruments. They provide AST results and resistance detection for clinically important Gram-positive cocci, Gram-negative bacilli and yeasts. The system is proven to be rapid, reliable and accurate.
How do you think the Vitek 2 instrument detects bacterial growth? VITEK® 2 continuously monitors growth in all wells. Growth in the positive control well is monitored until a pre-determined minimum amount of bacterial growth is detected through turbidity measurements (i.e. percent change of raw transmittance units – %ΔRTU).
